Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds

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Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds

AIM The present study was thus undertaken to analyze the genetic diversity of Kilakarsal and Vembur sheep breeds using single-nucleotide polymorphism (SNP) markers within Toll-like receptor (TLR) 3, 5, 6, 9, and 10 genes. MATERIALS AND METHODS Competitive allele-specific polymerase chain reaction (PCR)-based end-point genotyping was performed using real-time PCR to type the SNPs. Allele discr...

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Application of single-nucleotide polymorphism (SNP) as a molecular marker in the study of genetic diversity of aquatic populations

Genetic diversity is one of the important and essential characteristics of any population for its survival. The study of genetic variation in different populations of aquatic organisms is of particular importance in order to protect, stabilize and manage their stocks. Based on studies conducted in recent years, molecular markers have proven that they can be used as indicators of the genetic div...

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Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers

The genetic diversity in the Kilakarsal sheep population was analysed using 25 microsatellite markers by multiplex PCR in five panels and automated DNA sequencer. The number of alleles ranged from 3 to 13 with a mean of 7.6 across loci. The mean effective number of alleles was 3.88. The size and frequencies of the alleles ranged from 80 to 278 bp and from 0.0100 to 0.8000, respectively. The PIC...

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Background Single nucleotide polymorphism (SNPs) are considered as one of the underlying causes of male infertility. Proper sperm chromatin packaging which involves replacement of histones with protamines has profound effect on male fertility. Over 20 SNPs have been reported for the protamine 1 and 2. MaterialsAndMethods The aim of this study was to evaluate the frequency of two previously repo...

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ژورنال

عنوان ژورنال: Veterinary World

سال: 2017

ISSN: 0972-8988,2231-0916

DOI: 10.14202/vetworld.2017.549-555